This paper develops indicators for entrepreneurial culture and investigates their effect on the rate of nascent entrepreneurship. We choose three measures from the World Value Survey. These include two desired job characteristics, i.e. the opportunity to use initiative and the possibility to achieve something, as well as an indicator of internal locus of control as our indicators for entrepreneurial culture. Controlling for economic, institutional and demographic determinants of nascent entrepreneurship we find a positive and significant relationship between entrepreneurial culture and nascent entrepreneurship.